Microfluidic probe for advanced staining of human tissue sections

A key challenge in diagnostic pathology is to provide accurate and precise diagnostics with limited excised tissue. It is also increasingly important to test the tissue samples against multiple markers to enhance the confidence in results and sub-type the diseases for personalized treatment therapies. To this end, we performed local chemical reactions and staining on tissue sections using a scanning microfluidic probe (MFP). With the MFP, human thyroid tissue sections were locally treated with anti-thyroglobulin and counterstains, enabling the possibility to optimize in real-time the staining conditions individually for each tissue/antibody pair. Copyright © (2011) by the Chemical and Biological Microsystems Society.