Evaluation of stained and unstained electropherograms by means of photoacoustic spectroscopy

Abstract

It is demonstrated that photoacoustic spectroscopy is a superior tool in the evaluation of electropherograms. The locla distribution of proteins in ultrathin layers of polyacrylamide gels used for isoelectric focusing is mapped with a resolution equal to or better than a laster densitomer. Even unstained samples of colored proteins can be mapped with excellent sensitivity and resolution due to the fact that the photoacoustic effect is caused only by the absorbed fraction of the incident light; scapttered light therefore doesse not cause a signal. in situ photoacosutic spectra of previously localzed zones allow the identification of the prosthetic groups of these proteins. Copyright © 1983 Verlag Chemie, GmbH