Size-based biomolecular separation enabled by field-effect electroosmosis

Abstract

We present a novel separation method that utilizes bidirectional flow to achieve separation of molecules based on their diffusivity. We use an array of gate electrodes actuated by alternate-current field-effect electroosmosis (ac-FEEO) to generate the bidirectional flow, and as a proof-of-concept demonstrate the separation of labeled antibodies from free labeling dye.