Biointegrated Subtractive Microfabrication by Hydrodynamic Flow Confinement

We present a new method that leverages hydrodynamic flow confinement to achieve both mechanical milling of micro-channels and patterning of biological molecules in a single process. We demonstrate applicability of the method through milling of a microchannel in polystyrene substrate through confinement of dichloromethane, followed by patterning of streptavidin in desired spots along the channel. This work is a first step toward our goal of achieving fully bio-integrated prototyping.